. Author manuscript; available in PMC: 2012 Sep 5.

Published in final edited form as: Bioconjug Chem. 1992 May-Jun;3(3):234–240. doi: 10.1021/bc00015a006

Table II.

Characterization of Compounds Synthesized

compd^e	formula	R_f		low resolution^a	high resolution^a
compd^e	formula	A^d	B^d	low resolution^a	calcd	found
3	C₂₈H₃₉BrN₄O₂S	0.66		577,^b 575,^b 497, 317, 185	575.2055	575.2027
					577.2038	577.2029
4	C₂₈H₄₁N₅O₂S	0.15	0	512,^b M⁺ (511),^c 368,^c 327,^c 314,^c 254^c	512.3059	512.3073
5	C₃₇H₄₉N₅O₄S	0.51	0.53	660,^b 402, 277,185	660.3584	660.3607
6	C₃₆H₄₈N₆O₃S	0.41	0.32	645,^b 387, 318, 229	645.3584	645.3608
8	C₃₅H₄₄N₈O₄S	0.69	0.75	674,^b 647, 553, 461, 369, 277	673.3284	673.3344
9	C₃₈H₅₅N₇O₄S₂	0.39	0.26	738,^b 468, 342, 227	738.3835	738.3848
10	C₃₆H₄₅N₇O₂S₃	0.83	0.93	704,^b 688, 307, 242	704.2875	704.2894
11	C₃₇H₄₆N₈O₃S₃		0.58	747,^b 461, 369, 277	747.2933	747.2964
12	C₃₇H₄₄N₈O₂S₄	0.85	0.9	761,^b 729, 553, 461, 369	761.2548	761.2570
13	C₃₆H₄₅N₇O₂S₃	0.89	0.94	704, 553, 461, 369, 277	704.2875	704.2891
14	C₄₉H₅₁N₅O₈S	0	0.31	870,^b 613, 461, 309	870.3534	870.3535
15	C₄₉H₄₈Br₄N₆O₇S₂		0.30	1217^b	1212.9838	1212.984

Unless noted, by fast atom bombardment mass spectroscopy, using glycerol matrix, in the positive ion mode.

M + 1.

EI mass spectroscopy.

Solvent A = chloroform/methanol/ammonia, 90:10:1; solvent B = chloroform/methanol/acetic acid, 85:10:5.

Refer to Table I for structures. Yields for conjugates of TAC (compounds 5 and 7–13) were generally in the range of 50–80%.