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. Author manuscript; available in PMC: 2012 Sep 5.
Published in final edited form as: Bioconjug Chem. 1992 May-Jun;3(3):234–240. doi: 10.1021/bc00015a006

Table III.

Irreversible Inhibition of Rat Muscarinic Receptors Resulting from Incubation of Membranes with an Isothiocyanate Derivative at a Concentration of 100 nM

compd % decrease in bindinga
brain heart
10 43 11
12 12 15
13 62 32
a

Decrease in specific binding of a single concentration (0.50 nM) of [3H]N-methylscopolamine from rat forebrain or rat heart membranes. Membranes were incubated with the indicated isothiocyanate derivative, freshly diluted from a DMSO stock solution (stored at −20 °C) into phosphate-buffered saline (pH 8.0,10 mM phosphate, 0.15 M sodium chloride) for 60 min at room temperature followed by radioligand binding.