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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Biochem Pharmacol. 2012 Jul 22;84(7):936–948. doi: 10.1016/j.bcp.2012.07.010

Fig. 1.

Fig. 1

Comparison of the affinity of [D-Tyr6, β-Ala11, Phe13, Nle14]Bn(6-14) [Univ.Lig] for all human BnR’s and hCCKAR. CHOP cells were transiently transfected with hGRPR, hNMBR, hBRS-3 or hCCKAR as described in Methods. Subsequently, the transfected cells were incubated with 50 pM 125I- [D-Tyr6, β-Ala11, Phe13, Nle14]Bn(6-14) [Univ.Lig] for 1 h at 21°C with or without various concentrations of [D-Tyr6, β-Ala11, Phe13, Nle14]Bn(6-14) [Univ.Lig]. Saturable binding was determined as described in Methods, and the results are expressed as the percentage of saturable binding without [D-Tyr6, β-Ala11, Phe13, Nle14]Bn(6-14) [Univ.Lig] present. Results are mean ± SEM of at least 3 separate experiments and each point was determined in duplicate.