Fig. 2.

Transmembrane hydropathy plot of the hGRPR. Transmembrane hydropathy plot of the hGRPR showing amino acid residues examined by site-directed mutagenesis in the present study. The transmembrane areas of the hGRPR were identified by hydropathy plots as described in Methods. This diagram shows the extracellular side above, and the cytoplasmic side below, with the three glycosylation sites shown by the Y-like symbols. The 74 amino acids identified as described in Methods from comparative alignment of bombesin receptors as possibly having an important role in determining high affinity for Univ.lig. and that were mutated in the present study are shown in the black circles.