Fig. 2.

Activation of p38MAPK in DNA-damage-induced senescence contributes to suppression of HSR. (A) Early passage cells were pre-treated with p38MAPK inhibitor SB for 6 h prior to 10 Gy IR. After IR, cells were incubated for 6 days before collection. Levels of p–p38 and p-Hsp27 were measured by immunoblotting. (B) Hsp70 was measured after heat shock in cells with overexpression of p16. (C) Same cells as from (A) were heat shocked and incubated for 6 h before collection. Addition of SB led to partial restoration of Hsp70 induction level after heat shock.