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. Author manuscript; available in PMC: 2013 Oct 11.
Published in final edited form as: Neuroscience. 2012 Jul 27;222:147–158. doi: 10.1016/j.neuroscience.2012.07.033

Figure 3.

Figure 3

Effects of exposure to the glutamate NMDA receptor agonist NMDA or co-exposure to NMDA and the competitive antagonist APV on immunoreactivity for the synaptic vesicle protein synaptophysin (SYN) observed in the organotypic hippocampal slice cultures. (A) Exposure to NMDA resulted in significant time-dependent synaptophysin loss within the CA1 region of the hippocampus, an effect which was prevented by co-exposure to APV. Collapsed across time, NMDA exposure resulted in significant decreased synaptophysin immunoreactivity within the CA3 (B) and DG (C) regions of the hippocampus. * p<0.05 vs. control levels; # p<0.05 vs. NMDA alone. (D) Representative images of immunoreactivity for synaptophysin in hippocampal slices treated with NMDA or NMDA+APV