Figure 8.

Secondary passage of RML^Δ23–31 scrapie into Tg(Δ23–31) mice does not shorten survival times. A, Survival times were monitored in mice of the following genotypes inoculated with RML or RML^Δ23–31: Tg(Δ23–31^6×) (red lines); Tga20^+/+ (black lines); non-Tg (gray lines). The circles represent individual mice inoculated with RML, and the squares represent mice inoculated with RML^Δ23–31. A statistical analysis of these data is shown in Table 3. B, Brain homogenates from terminally ill mice of the indicated genotypes inoculated with RML (lanes 1–3) or RML^Δ23–31 (lanes 4–15) were treated without (top panel) or with (bottom panel) PK, and were then subjected to Western blotting using anti-PrP antibody 6D11. Actin is shown as a loading control.