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. Author manuscript; available in PMC: 2012 Sep 5.
Published in final edited form as: Leukemia. 2011 Oct 28;26(5):1122–1127. doi: 10.1038/leu.2011.309

Figure 3.

Figure 3

NF-κB2 shRNAs induced apoptosis in TRAF3–/– B-lymphoma cells. Mouse B-lymphoma cells (M12.4.1 cell line in (a), and TRAF3–/– B lymphoma cell line 27-9.5.3 in (b, c, d)) were transduced with lentiviruses expressing mouse NF-κB2 shRNAs or a scrambled shRNA (SCR). (a) Total cellular protein levels of p100 and p52 NF-κB2 determined by immunoblot analysis. Proteins were prepared from B-lymphoma cells transduced with a scrambled shRNA, or NF-κB2 shRNA 653, 1226, 2716 or 2996. Immunoblot of actin was used as loading control. (b) Growth curves of live cells and (c) percentage of live versus dead cells determined by trypan blue-stained cell counting. The graphs depict the results of three independent experiments (mean ± s.d.). (d) Representative FACS profiles of transduced cells analyzed by annexin V and propidium iodide (PI) staining. Apoptotic cells were identified as annexin V+ PI–, dead cells were annexin V+ PI+ and live cells were annexin V– PI–. Data are representative of three independent experiments.