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. 2012 Aug 3;31(17):3550–3563. doi: 10.1038/emboj.2012.193

Figure 1.

Figure 1

Transcription inhibition does not influence UV-induced TFIIH recruitment in XP-D/CS cells. (A) Scheme of the murine construction used (left), the fluorescent mutated complex produced in the mouse model (centre) and a picture of a double homozygote mouse XpbY/Y·XpdG602D (noted XPBYFPXPCS) (right). (B) Confocal images of FRAP procedure on a LD area in murine keratinocyte isolated from the XpbY/Y mouse model. Cells were damaged with UV (60 J/m2) through filters to create local XPBYFP accumulations, then XPBYFP mobility was measured by FRAP on the local damages. Scale bar, 5 μm. (C) FRAP on local damage curves of XPBYFP (green) and XPBYFPXPCS mouse keratinocytes treated (blue) or not (red) with a 16-h incubation of α-amanitin before the local UV irradiation. Vertical bars represent the standard error of the mean (s.e.m.). (D) Bar graph showing the average binding of TFIIH on four different active promoter regions, untreated (−UV in blue) and 1/2 h after 20 J/m2 global UVC exposure (+UV in red). Values are normalized to an intergenic region and the vertical bars represent the s.e.m. of the four promoters.