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. 2012 Aug 3;31(17):3550–3563. doi: 10.1038/emboj.2012.193

Figure 2.

Figure 2

Effects of transcription inhibition on H2AX phosphorylation and RPA recruitment to DNA damage are independent of the XP-D/CS mutation. (A) Images of γH2AX (green) and XPB (red) immunostained cells 1 h after local UV irradiation (60 J/m2). Cells were untreated (columns 1 and 2), treated for 6 h with α-amanitin (columns 3 and 4) or for 16 h with α-amanitin (columns 5 and 6) prior to UV exposure. (B) Images of RPA (green) and XPB (red) immunostained cells 1 h after local UV irradiation (60 J/m2). Cells were untreated (columns 1 and 2), treated for 6 h with α-amanitin (columns 3 and 4) or treated for 16 h with α-amanitin (columns 5 and 6) prior to UV exposure. All scale bars, 5 μm.