Figure 3.
Transcription inhibition does not affect XPF and XPG recruitment to DNA damage sites. (A) Images of XPF (green) and XPB (red) immunostained cells 1 h after local UV irradiation (60 J/m2). Cells were untreated (columns 1 and 2), treated for 6 h with α-amanitin (columns 3 and 4) or treated for 16 h with α-amanitin (columns 5 and 6) prior to UV exposure. (B) Images of XPG (green) and XPB (red) immunostained cells 1 h after local UV irradiation (60 J/m2). Cells were untreated (columns 1 and 2), treated for 6 h with α-amanitin (columns 3 and 4) or treated for 16 h with α-amanitin (columns 5 and 6) prior to UV exposure. All scale bars, 5 μm.