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. 2012 Jul 6;31(17):3513–3523. doi: 10.1038/emboj.2012.183

Figure 3.

Figure 3

Tumour secreted miR-9 promotes endothelial cell migration. (A) HUVEC migration in response to SK23 conditioned medium, SK23 microvesicles or HM7 microvesicles was analysed by xCELLigence system. Each group had three biological replicates. The experiment was repeated twice. For statistical analysis, ANOVA was performed followed by Tukey’s post-test to compare two groups. (B) SK23 cells were transfected with scrambled or Drosha siRNAs and purified microvesicles were tested to induce HUVEC migration. Each group had five biological replicates. (C) HUVECs were transfected with miR-9 or miR-9* and stained by crystal violet or FITC-phalloidin. (D) Cell migration of HUVECs overexpressing miR-9. Each group had three biological replicates. The experiment was repeated three times. ANOVA was performed followed by Tukey’s post-test to compare two groups (P<0.05). (E) HUVECs were transfected with miR-9 antagomirs and cell migration stimulated by SK23 microvesicles was assessed. Each group had three biological replicates. The experiment was repeated twice. ANOVA was performed followed by Tukey’s post-test to compare two groups (P<0.05). (F) Phalloidin staining of HUVEC sprouts and quantification of mean sprouting length per bead. *P<0.05, Student’s t-test.