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5-Thiomannosides decrease the extent of N-glycosylation observed in CHO K1 cells. Western blots of cell lysates isolated from treated cells were probed with the lectins (A) ConA, (B) DSA, and (C) GNA. Protein concentrations were measured and equal amounts of protein were added to each lane. The high-mannose-specific glycosidase EndoH was used only of GNA.
