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. Author manuscript; available in PMC: 2012 Sep 5.
Published in final edited form as: Org Biomol Chem. 2011 Aug 16;9(19):6513–6518. doi: 10.1039/c1ob05874f

Fig. 3.

Fig. 3

Delivery of streptavidin-Texas Red® into Clone 9 cells at 37 °C mediated by 1 and 2. Data for transport using polymer 1: (a) polymer 1 channel; (b) streptavidin-Texas Red® channel; (c) LysoTracker® Green; (d) colocalization shows mostly distinct blue, red, and green areas. Data for transport using polymer 2: (e) polymer 2 channel; (f) streptavidin-Texas Red® channel; (g) LysoTracker® Green; (h) colocalization shows mainly white areas where all three labels coexist. Throughout, the carrier (1.0 μM), streptavidin-Texas Red® (1.0 μM), and the Clone 9 cells were incubated at 37 °C for 15 h; the cells were then washed 3× with PBS buffer and 3× with heparin and analysed via fluorescence microscopy. Scale bar is 20 μm.