Table 1.
Antibodies used for flow cytometry and for immunofluorescence
| Antibodies with conjugate | Vendor and catalogue no. | Clone | Volume/106 cells or dilutions used |
|---|---|---|---|
| CD34-PE | BD Biosciences #550619 | 563 | 10 μl |
| CD31-FITC | BD Biosciences #557508 | WM59 | 5 μl |
| CD117-APC | BD Biosciences #550412 | YB5.B8 | 5 μl |
| CD45-PerCP | BD Biosciences #558411 | DO58-1283 | 5 μl |
| CD146-PE | R&D Systems FAB932P | 12808 | 10 μl |
| CD54-APC | BD Biosciences #559771 | HA58 | 10 μl |
| CXCR4-APC | BioLegend #306510 | 12G5 | 5 μl |
| CD181-FITC | BioLegend #320605 | 8F1 | 5 μl |
| UEA-1-FITC | Sigma #9006 | 3 μl | |
| CD195-APC | BD Biosciences #550856 | 3A9 | 5 μl |
| VEGFR3-PE | R&D Systems #FAB3492P | 54733 | 5 μl |
| CD14-FITC | Beckman-Coulter #6603511 | MY4 | 5 μl |
| CD144 (VE-Cadherin) | Cell Signaling #2500 | Rabbit IgG | 1:400 |
| CD62E-FITC | R&D Systems #BBA21 | BB1G-E5 | 1:50 |
| Anti-rabbit with Texas Red | Santa Cruz #sc-2780 | Goat IgG | 1:400 |