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. Author manuscript; available in PMC: 2013 Apr 15.
Published in final edited form as: J Neurosci Methods. 2012 Jan 20;205(2):277–282. doi: 10.1016/j.jneumeth.2012.01.007

Figure 4. Characterization of primary cultures.

Figure 4

A-D. IHC for various neuronal and non-neuronal markers demonstrate the presence of MNs (Islet-1/2; A, A’(inset of boxed region in A)), synaptic vesicles (SV2; B), glia (GFAP; C) and muscle fibers (F59; D) in primary cultures from 48 hpf HB9:mGFP embryos. E-F. Utilization of MLS-EGFP transgenic zebrafish for primary cultures enables visualization of mitochondria in cell bodies and axons (arrowheads; E) of neurons, as demonstrated by IHC for a neuronal marker (NF; F). GFP expression in transgenic zebrafish lines in MNs is under control of the HB9 promoter (HB9:mGFP; A-D) or in mitochondria using a mitochondrial-localization signal (MLS-EGFP; E-F). Images are acquired using an Olympus BX-51 confocal microscope at 60X magnification. Scale bar equals 20 μm.