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Cryo-EM reconstruction of the reconstituted bovine 12-subunit RNAPII elongation complex. Three views of the cryo-EM reconstruction of bovine RNAPII elongation complex at ∼19 Å resolution (FSC 0.15). Superimposed with the EM envelop is the X-ray structure of yeast 12-subunit RNAPII (coloured ribbons) (PDB: 1Y1W). The threshold for rendering the EM reconstruction is chosen based on a molecular weight of ∼520 kDa.
