Fig. 3.

Quantitative RT-PCR (qRT-PCR) confirms microarray findings. A: the ratio of normalized transcription level on PID 4 relative to PID 0, of select genes identified as differentially expressed by microarray, were validated by quantitative RT-PCR. These genes were caspase 4 (CASP4), chemokine (C-C motif) ligand 3 (CCL3), CD40 antigen (CD40), guanylate nucleotide binding protein 2 (GBP2), interferon inducible GTPase 2 (IIGP2), interferon regulatory factor 8 (IRF8), SAM domain and HD domain 1 (SAMHD1), and tumor necrosis factor (ligand) superfamily, member 10 (TNFSF10). For each gene in the qRT-PCR assay, the three biological replicates were analyzed in duplicates and normalized to the L19 mean value. Shown are means ± SE. B: scatter plot representation of the ratio of qRT-PCR data to microarray data (R² = 0.86).