Table 2.
Effects of dopamine and PKA inhibitors and bafilomycin on flow-induced changes of Jv under low and high perfusion rates in mouse proximal tubules
| 5 nl/min |
20 nl/min |
|||||
|---|---|---|---|---|---|---|
| Group | n | JVa, nl·min−1·mm−1 | n | JVb, nl·min−1·mm−1 | ΔJV (JVb − JVa) | ΔJV/JVa * 100 |
| Control | 14 | 0.88 ± 0.03 | 15 | 1.35 ± 0.04c | 0.48 ± 0.05 | 54.31 ± 4.10 |
| Dopamine | 9 | 0.85 ± 0.08NS | 12 | 0.92 ± 0.06ns,C | 0.07 ± 0.10C | 7.69 ± 7.53C |
| DA + bafilomycin | 7 | 0.84 ± 0.05NS | 8 | 0.96 ± 0.04ns,C | 0.12 ± 0.06C | 14.05 ± 5.3C |
| DA + SCH | 11 | 0.97 ± 0.04NS | 13 | 1.73 ± 0.05c,C | 0.77 ± 0.06C | 79.53 ± 5.20C |
| DA + Sul | 10 | 0.83 ± 0.06NS | 12 | 1.12 ± 0.04c,C | 0.28 ± 0.07A | 33.88 ± 4.28B |
| DA + SCH + Sul | 7 | 1.12 ± 0.13A | 13 | 1.66 ± 0.14a,A | 0.54 ± 0.19NS | 48.50 ± 12.20NS |
| 8-Bromo-cAMP | 7 | 0.69 ± 0.03C | 10 | 1.02 ± 0.04c,C | 0.33 ± 0.05NS | 46.96 ± 5.26NS |
| DA + H89 | 10 | 0.82 ± 0.09NS | 13 | 1.44 ± 0.06c,NS | 0.62 ± 0.11NS | 75.12 ± 6.81B |
| SCH | 6 | 0.79 ± 0.04NS | 8 | 1.36 ± 0.03c,NS | 0.57 ± 0.05NS | 72.34 ± 3.56B |
Values are means ± SE; n = number of perfused tubules. Jv, rate of fluid reabsorption; ΔJv, differences of Jv between low (JVa) and high (JVb) perfusion rates (5 and 20 nl/min); ΔJv/Jva * 100, percent changes in fluid reabsorption from low flow rate; Sul, sulpiride; SCH, SCH23390. ns: not significantly different from low flow rate in the same group; a,b,csignificant difference from low flow rate in the same group (aP < 0.05; bP < 0.01; cP < 0.001). NS: nonsignificant difference compared with control at the similar flow rate; A,B,Csignificant difference compared with control at the similar flow rate (AP < 0.05; BP < 0.01; CP < 0.001).