Table 5.
Effects of dopamine and PKA inhibitors and bafilomycin on chloride absorption under low and high perfusion rates in mouse proximal tubules
| 5 nl/min |
20 nl/min |
|||||
|---|---|---|---|---|---|---|
| Group | n | JCla, pmol·min−1·mm−1 | n | JClb, pmol·min−1·mm−1 | ΔJCl (JClb − JCla) | ΔJCl/JCla * 100 |
| Control | 14 | 66.79 ± 1.58 | 15 | 69.79 ± 6.99ns | 2.99 ± 7.28 | 4.48 ± 10.47 |
| Dopamine | 9 | 63.71 ± 7.72NS | 12 | 32.21 ± 5.87b,e | −31.49 ± 9.51d | −49.43 ± 9.21e |
| DA + bafilomycin | 7 | 76.28 ± 6.69NS | 8 | 83.42 ± 5.48ns,NS | 7.14 ± 8.59NS | 9.36 ± 7.18NS |
| DA + SCH | 11 | 82.59 ± 4.19e | 13 | 127.65 ± 5.35b,e | 45.06 ± 6.86e | 54.56 ± 6.48e |
| DA + Sul | 10 | 69.29 ± 7.54NS | 12 | 52.84 ± 5.1ns,NS | −16.45 ± 8.95NS | −23.74 ± 7.38c |
| DA + SCH + Sul | 7 | 83.76 ± 15.72NS | 13 | 96.60 ± 15.20ns,NS | 12.84 ± 21.76NS | 15.33 ± 18.14 NS |
| 8-Bromo-cAMP | 7 | 49.80 ± 7.31d | 10 | 42.45 ± 5.79ns,c | −7.35 ± 9.13NS | −14.76 ± 11.62NS |
| DA + H89 | 10 | 60.57 ± 12.45 NS | 13 | 75.96 ± 7.41ns,NS | 15.39 ± 14.03NS | 25.42 ± 12.23NS |
| SCH | 6 | 47.77 ± 5.92e | 8 | 63.53 ± 3.97a,NS | 15.76 ± 6.93NS | 32.99 ± 8.30NS |
Values are means ± SE; n = number of perfused tubules; JCl: the rate of chloride reabsorption; ΔJCl: the differences of JCl between low (JCla) and high (JClb) perfusion rates (5 and 20 nl/min); ΔJCl/ JClb*100: percentage changes in chloride reabsorption from low flow rate; ns, not significant different from low flow rates in the same group; a,bsignificant difference from low flow rate in the same group (aP < 0.05; bP < 0.001). NS, not significant different compared with control at the similar flow rates; c,d,esignificant difference compared with control at the similar flow rates (cP < 0.05; dP < 0.01; eP < 0.001).