Table 1.
Primers used for quantitative real-time PCR (qRT-PCR)
| Gene | Gene ID | Forward | Reverse |
|---|---|---|---|
| AGT | NM_134432 | 5′-CCCTGAGCAGTCCGTTCCT-3′ | 5′-AAAGTGCAGCGCACCTGAGT-3′ |
| AT1A | NM_030985 | 5′-CAAGTCCCACTCAAGCCTGTC-3′ | 5′-TGTTATCCGAAGGCCGGTAA-3′ |
| AT1B | NM_031009 | 5′-CCTCCGCCGCACGAT-3′ | 5′-CCAGCCATTAGCCAGATGATG-3′ |
| AT2 | NM_012494 | 5′-TGCTGTTGTGTTGGCATTCA-3′ | 5′-ATCCAAGAAGGTCAGAACATGGA-3′ |
| ACE 1 | NM_012544 | 5′-TTTGCTACACAAATGGCACTTGT-3′ | 5′-CGGGACGTGGCCATTATATT-3′ |
| ACE 2 | NM_001012006 | 5′-TTGAACCAGGATTGGACGAAA-3′ | 5′-GCCCAGAGCCTACGATTGTAGT-3′ |
| Renin | NM_012642 | 5′-CAGGAACGATGACCTGTGCAT-3′ | 5′-CAGTGGGTGGTGGGATGTC-3′ |
| MasR | NM_001001506 | 5′-CACTGGCCCTCCTGATGAA-3′ | 5′-GGATGCCAGAATTGAACACAGA-3′ |
| 18 S | EU139318 | 5′-GTAGTCGCCGTGCCTACCAT-3′ | 5′-TCCGGAATCGAACCCTGA T-3′ |
| β-Actin | AF541940 | 5′-GGCCCAGTACGGCACAGT-3′ | 5′-CCACCGCCGTCTCACTCT-3′ |
AT1A, AT1B, and AT2: ANG II subtypes; ACE, angiotensin-converting enzyme; MasR, Mas receptor.