Fig. 4.

Metabolite signatures of GBM subtypes and correlations with clinical parameters. A, Number of metabolites that change in the CSF of malignant glioma samples relative to the control patients as a function of p value cutoff using a two-tailed t test statistic. B, TCA cycle signature associated with patients 3 and 10 and its relative contribution across patient samples. Signature score is defined as the normalized sum of metabolite intensity of the citric acid cycle components (succinate, fumarate, alpha-ketoglutarate, malate, oxaloacetate, isocitrate, and citrate). C, Tryptophan metabolism intermediates associated with recurrent disease (p value < 0.05). D, Example metabolites cholesteryl-sulfate and myo-inositol that positively and negatively correlate (R²) with tumor size as measured with MRI using Tgad and FLAIR (x axis) and correlated with the integrated total ion current (TIC) peak areas (y axis) obtained from targeted LC-MS/MS measurements.