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Modulation of La/SSB expression affects Nrf2 protein level. HeLa cells were transfected with siRNA against La/SSB (A), negative control siRNA (A) or GFP-La/SSB expression vector (B) before treatment with 100 μm H2O2 for 10 min at 24 h after transfection. The cells were harvested 1 h later for Western blot analysis of La/SSB and Nrf2 with vinculin as a loading control.
