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Subcellular distribution of La/SSB protein. HeLa cells were treated with H2O2 at indicated doses and harvested 1 h later. Levels of La/SSB or Nrf2 protein in total cell lysates were measured using Western blot with vinculin as the loading control (A). Cytoplasmic and ribosomal fractions were isolated as described in the Method for measurements of La/SSB protein levels by Western blot (B). Ribosomal protein L36a was used as the loading control for ribosomal fractions.
