Fig. 3.

Strategy for identification of highly specific mAbs. Various different live human cell lines were used for immunization of BALB/c mice. The resulting hybridomas were tested for secretion of IgG and were used for ICC for the cell lines used. ICC-positive supernatants were combined in 12 × 12 two-dimensional pools, which were then used to probe the human proteome microarrays. Data from pooled samples were deconvoluted, and the candidate monospecific mAbs were then probed to the human proteome microarrays individually. Examples of antigens recognized by monospecific mAbs are shown.