Table II. Candidate urinary biomarkers for sepsis-induced acute kidney injury: selection approaches based on quantitative gel-free proteomics data. Accession numbers of identified proteins are shown as provided in the UniProt database. Data of the quantitative comparison of urinary proteomes collected before (T0) and at 48 h after infection (T1) from mice which were septic (S) or were septic and developed AKI at T1 (S+AKI) are represented. Data are shown from proteins selected by approach 1 (n = 15, italics type font), approach 1 bis (n = 14, regular type font), and in both approach 1 and 1 bis (n = 10 bold type font) for which a commercial antibody is available. Blank fields link to proteins that were not detected in all three technical repeats of one analysis. ID: number of identified peptides, different peptides: number of identified unique peptides.

Protein	Accession number	S+AKI T1/S T1			S+AKI T1/T0			S T1/T0
		Fold increase at T1 in S+AKI compared to S	ID	Different peptides	Fold increase at T1 compared to T0	ID	Different peptides	Fold increase at T1 compared to T0	ID	Different peptides
Neutrophil gelatinase-associated lipocalin	P11672	3.7	38	5	714.3	22	4	120.9	16	3
Uteroglobin	Q06318	5.5	16	3	400.0	13	3	2.4	5	1
Osteopontin	P10923	5.3	179	13	17.2	167	13	35.0	73	10
Thioredoxin	P10639	2.5	15	3	11.9	9	3	30	6	2
Protein S100-A8	P27005	20.6	6	1
Parvalbumin alpha	P32848	17.7	32	11	212.8	43	12
Myoglobin	P04247	15.7	11	4	416.7	12	3
Annexin A1	P10107	7.1	3	1	5.7	3	2
Lactotransferrin	P08071	4.3	3	2	8.0	5	3
Apolipoprotein A-I	Q00623	4.0	5	2	2.7	5	3
Peptidyl-prolyl cis-trans isomerase A	P17742	3.9	4	2	27.9	6	3
Meprin A subunit beta	Q61847	3.3	4	2
Cathepsin D	P18242	3.1	4	2	23.1	4	2
Calbindin	P12658	3.1	6	1
Ig kappa chain C region	P01837	2.4	11	4	14.4	20	4	14.7	13	5
Ezrin	P26040	2.4	20	5	9.5	25	9	5000.1	11	6
Gamma-glutamyltranspeptidase 1	Q60928	2.4	19	4	15.2	13	5	14.1	7	3
Lymphocyte antigen 6D	P35459	2.4	7	1	11.6	9	1	43.1	4	1
Na(+)/H(+) exchange regulatory cofactor NHE-RF3	Q9JIL4	2.4	6	3	10.5	13	5	6.8	12	6
Insulin-like growth factor-binding protein 7	Q61581	2.3	5	1	11.3	4	2
Serotransferrin	Q921I1	2.3	85	13	18.0	89	18	38.3	37	12
Vitamin d-binding protein	P21614	2.2	13	5	25.0	13	7	37.9	11	6
Alpha-enolase	P17182	2.1	9	5	14.5	11	5
Transthyretin	P07309	2.1	6	1	6.1	15	4	18.1	5	1
Sepiapterin reductase	Q64105				35.5	3	1
Gelsolin	P13020				34.5	5	2
Cathepsin L1	P06797				24.8	5	2
Chitinase-3-like protein 3	O35744				21.6	9	3
Lipopolysaccharide-binding protein	Q61805				117.6	3	1
Complement C3	P01027				19.6	12	8
Serotransferrin	Q921I1	2.3	85	13	18.0	89	18	38.3	37	12
N-acetylglucosamine-6-sulfatase	Q8BFR4				18.2	9	5
Prothymosin alpha	P26350				15.7	3	1
Annexin A5	P48036				13.0	10	3
UMP-CMP kinase	Q9DBP5				12.9	3	1
Beta-2-microglobulin	P01887				11.5	3	1
kinesin-associated protein 3	P70188				10.7	4	1
Destrin	Q9R0P5				10.4	3	1
Heat shock cognate 71 kDa protein	P63017				9.8	4	2

12C sample	13C sample
S+AKI T0	S+AKI T1
S T1	S T0
S T1	S+AKI T1