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. Author manuscript; available in PMC: 2012 Sep 5.
Published in final edited form as: Cancer Gene Ther. 2007 Nov 9;15(2):61–72. doi: 10.1038/sj.cgt.7701107

Figure 5.

Figure 5

Influence of TRAIL on spread and extracellular yields of oncolytic Ad. (a) High level of TRAIL expression impairs TetON–TRAIL spread in 293 cells; low basal expression improves the spread. 293 cells were infected with TetON–TRAIL or TetON–EGFP at various MOI. Transgene expression was induced by Dox. Cell viability was quantified at day 6 postinfection. (b) Expression of TRAIL increases the extracellular titers of the tumor-specific oncolytic Ad in Hep3B cells. Cells were coinfected with TetON–TRAIL and KD3, or TetON–EGFP and KD3, at an MOI of 10PFU per cell. Transgene expression was induced by addition of Dox to the medium at 0 or 24 h postinfection. Virus titers were determined in the medium at 48 h postinfection. EGFP, enhanced green fluorescent protein; MOI, multiplicity of infection; PFU, plaque forming unit; TRAIL, tumor necrosis factor-related apoptosis-inducing ligand.