Appendix 1.
Species, population, locus, and number of samples (N), alleles (A), expected (He), and observed (HO) heterozygosity. Inbreeding coefficient (FIS), null alleles (Null), corrected inbreeding coefficient (FISC). Large allele drop-out (LAD), not enough data (NED), not applicable (NA). Asterisks indicate significant deviations from Hardy–Weinberg equilibrium (P < 0.05).
Locus | N | A | He | HO | FIS | Null | FISC | ||
---|---|---|---|---|---|---|---|---|---|
Acropora microphthalma | Kimbe Bay | 2 | 23 | 12 | 0.849 | 0.826 | 0.105 | No | 0.105 |
6 | 23 | 5 | 0.691 | 0.783 | –0.147 | No | –0.147 | ||
28 | 23 | 6 | 0.509 | 0.435 | 0.096 | No | 0.096 | ||
22 | NA | NA | NA | NA | NA | No | NA | ||
23 | 23 | 6 | 0.778 | 0.652 | 0.136* | No | 0.136 | ||
7 | 22 | 6 | 0.478 | 0.045 | 0.92* | Yes | 0.119 | ||
10 | 25 | 10 | 0.728 | 0.72 | 0.031 | No | 0.031 | ||
12 | 25 | 6 | 0.406 | 0.32 | 0.232* | No | 0.232 | ||
Seychelles | 2 | 22 | 8 | 0.747 | 0.611 | 0.141 | No | 0.141 | |
6 | 22 | 7 | 0.638 | 0.529 | 0.191 | No | 0.191 | ||
28 | 22 | 20 | 0.787 | 0.389 | 0.515* | Yes | 0.169 | ||
22 | NA | NA | NA | NA | NA | NA | NA | ||
23 | 22 | 3 | 0.575 | 0.714 | –0.171 | No | –0.171 | ||
7 | 22 | 7 | 0.702 | 0.591 | 0.181* | No | 0.181 | ||
10 | 22 | 12 | 0.778 | 0.545 | 0.32* | Yes | 0.267 | ||
12 | 22 | 6 | 0.635 | 0.318 | 0.516* | Yes | 0.115 | ||
Maldives | 2 | 19 | 3 | 0.661 | 0.579 | 0.15 | No | 0.15 | |
6 | 19 | 6 | 0.726 | 0.789 | –0.061 | No | –0.061 | ||
28 | 19 | 7 | 0.74 | 0.684 | 0.102 | No | 0.068 | ||
22 | NA | NA | NA | NA | NA | NA | NA | ||
23 | 19 | 4 | 0.35 | 0.421 | –0.176 | No | –0.176 | ||
7 | 19 | 5 | 0.56 | 0.333 | 0.566 | Yes | 0.186 | ||
10 | 19 | 5 | 0.738 | 0.533 | 0.176* | Yes | 0.16 | ||
12 | 19 | 5 | 0.616 | 0.867 | –0.397 | No | –0.397 | ||
Orpheus Island | 2 | 22 | 6 | 0.624 | 0.773 | –0.216 | No | –0.216 | |
6 | 22 | 5 | 0.636 | 0.818 | –0.264 | No | –0.264 | ||
28 | 22 | 4 | 0.17 | 0.091 | 0.485 | Yes | –0.05 | ||
22 | NA | NA | NA | NA | NA | NA | NA | ||
23 | 22 | 4 | 0.458 | 0.273 | 0.423* | Yes | 0.133 | ||
7 | 22 | 5 | 0.522 | 0.409 | 0.238 | No | 0.238 | ||
10 | 22 | 6 | 0.667 | 0.864 | –0.273 | No | –0.273 | ||
12 | 22 | 6 | 0.674 | 0.909 | –0.329* | No | –0.329 | ||
A. valida | Orpheus Island | 2 | 28 | 6 | 0.596 | 0.357 | 0.281* | Yes | 0.028 |
6 | 25 | 6 | 0.728 | 0.52 | 0.238* | Yes | 0.105 | ||
28 | 26 | 13 | 0.877 | 0.654 | 0.231* | Yes | 0.225 | ||
22 | NA | NA | NA | NA | NA | NA | NA | ||
23 | 27 | 4 | 0.141 | 0.074 | 0.378 | Yes | 0.043 | ||
7 | 28 | 7 | 0.694 | 0.179 | 0* | No | 0 | ||
10 | 28 | 3 | 0.364 | 0.321 | 0.143 | No | 0.143 | ||
12 | 28 | 2 | 0.069 | 0 | 1* | No | 1 | ||
Heron Island | 2 | 17 | 7 | 0.78 | 0.588 | 0.223 | NED | 0.223 | |
6 | 13 | 3 | 0.506 | 0.077 | 0.727* | NED | 0.727 | ||
28 | 14 | 3 | 0.612 | 0 | 0.87* | NED | 0.87 | ||
22 | NA | NA | NA | NA | NA | NA | NA | ||
23 | 16 | 1 | 0 | 0 | 0 | NED | 0 | ||
7 | NA | NA | NA | NA | NA | NED | NA | ||
10 | 17 | 2 | 0.111 | 0.118 | –0.045 | NED | –0.045 | ||
12 | 17 | 1 | 0 | 0 | 0 | NED | 0 | ||
Kimbe Bay | 2 | 19 | 6 | 0.735 | 0.737 | 0 | No | 0 | |
6 | 19 | 6 | 0.719 | 0.368 | 0.535* | Yes | 0 | ||
28 | 19 | 15 | 0.904 | 0.421 | 0.571* | Yes | 0.085 | ||
22 | NA | NA | NA | NA | NA | No | NA | ||
23 | 19 | 4 | 0.389 | 0.368 | 0.089 | No | 0.089 | ||
7 | 19 | 4 | 0.609 | 0.105 | 0* | No | 0 | ||
10 | 19 | 5 | 0.503 | 0.368 | 0.3* | No | 0.3 | ||
12 | 19 | 2 | 0.188 | 0 | 1* | Yes | 0.3 | ||
A. austera | Majuro | 2 | 12 | 4 | 0.462 | 0.417 | 0.141 | No | 0.141 |
6 | 12 | 4 | 0.66 | 0.417 | 0.405 | No | 0.405 | ||
28 | 12 | 4 | 0.601 | 0.25 | 0.612* | Yes | 0.12 | ||
22 | NA | NA | NA | NA | NA | NA | NA | ||
23 | 11 | 1 | 0 | 0 | 0 | No | 0 | ||
7 | NA | NA | NA | NA | NA | No | NA | ||
10 | 12 | 1 | 0 | 0 | 0 | No | 0 | ||
12 | NA | NA | NA | NA | NA | NA | NA | ||
Arno | 2 | 14 | 5 | 0.666 | 0.857 | –0.253 | No | –0.253 | |
6 | 14 | 4 | 0.666 | 0.857 | –0.253 | No | –0.253 | ||
28 | 10 | 4 | 0.27 | 0.3 | –0.04 | No | –0.04 | ||
22 | NA | NA | NA | NA | NA | NA | NA | ||
23 | 14 | 2 | 0.191 | 0.214 | –0.083 | No | –0.083 | ||
7 | NA | NA | NA | NA | NA | NA | NA | ||
10 | 14 | 1 | 0 | 0 | 0 | No | NA | ||
12 | NA | NA | NA | NA | NA | NA | NA | ||
Maldives | 2 | 28 | 7 | 0.733 | 0.786 | –0.054 | No | –0.054 | |
6 | 28 | 6 | 0.625 | 0.536 | 0.161 | No | 0.161 | ||
28 | 28 | 3 | 0.307 | 0.286 | 0.087 | No | 0.087 | ||
22 | NA | NA | NA | NA | NA | NA | NA | ||
23 | 29 | 3 | 0.511 | 0.586 | –0.131 | No | –0.131 | ||
7 | NA | NA | NA | NA | NA | NA | NA | ||
10 | 29 | 6 | 0.728 | 0.655 | 0.118* | No | 0.118 | ||
12 | 29 | 6 | 0.702 | 0.448 | 0* | No | NA | ||
A. papillare | Ningaloo | 2 | 28 | 3 | 0.07 | 0.036 | 0.5* | Yes | –0.038 |
6 | 28 | 4 | 0.563 | 0.179 | 0.692* | Yes | –0.041 | ||
28 | 28 | 6 | 0.735 | 0.357 | 0.528* | Yes | 0.028 | ||
22 | 25 | 9 | 0.788 | 1 | –0.25* | No | –0.25 | ||
23 | 25 | 7 | 0.626 | 0.6 | 0.061 | No | 0.061 | ||
7 | NA | NA | NA | NA | NA | NA | NA | ||
10 | 28 | 7 | 0.721 | 0.571 | 0.225* | Yes | 0.015 | ||
12 | 26 | 5 | 0.57 | 0.462 | 0.208* | No | 0.208 | ||
Orpheus Island | 2 | 20 | 7 | 0.639 | 0.75 | 0.164 | No | 0.164 | |
6 | 20 | 3 | 0.411 | 0.2 | 0.532* | Yes | –0.282 | ||
28 | 20 | 8 | 0.8 | 0.8 | 0.026 | No | 0.026 | ||
22 | 20 | 14 | 0.814 | 1 | –0.204 | No | –0.204 | ||
23 | 20 | 5 | 0.585 | 0.15 | 0.755* | Yes | –0.078 | ||
7 | 20 | 3 | 0.421 | 0.15 | 0* | No | 0 | ||
10 | 20 | 7 | 0.706 | 0.75 | –0.036 | No | –0.036 | ||
12 | 20 | 4 | 0.554 | 0.8 | –0.424 | No | –0.424 | ||
Japan | 2 | 14 | 6 | 0.755 | 0.643 | 0.185* | No | 0.185 | |
6 | 14 | 4 | 0.543 | 0.571 | –0.015 | No | –0.015 | ||
28 | 14 | 4 | 0.707 | 0.071 | 0.906* | NED | 0.906 | ||
22 | 14 | 4 | 0.612 | 0.857 | –0.368 | No | –0.368 | ||
23 | 14 | 4 | 0.199 | 0.143 | 0.316 | No | 0.316 | ||
7 | 14 | 4 | 0.487 | 0.643 | 0 | No | 0 | ||
10 | 14 | 5 | 0.691 | 0.714 | 0.004 | No | 0.004 | ||
12 | 14 | 1 | 0 | 0 | 0 | No | 0 | ||
A. millepora | Orpheus Island | 2 | 26 | 3 | 0.556 | 0.385 | 0.326 | No | 0.326 |
6 | 26 | 4 | 0.499 | 0.385 | 0.248* | No | 0.248 | ||
28 | 26 | 4 | 0.362 | 0.192 | 0.485* | Yes | 0.162 | ||
22 | 26 | 16 | 0.873 | 0.846 | 0.05 | No | 0.05 | ||
23 | 26 | 3 | 0.473 | 0.308 | 0.367* | Yes | 0.006 | ||
7 | 18 | 3 | 0.642 | 0 | 1* | Yes | 1 | ||
10 | 26 | 13 | 0.749 | 0.731 | 0.044 | No | 0.044 | ||
12 | 26 | 2 | 0.074 | 0.077 | –0.02 | No | –0.02 | ||
Ningaloo | 2 | 32 | 5 | 0.409 | 0.469 | –0.131 | No | –0.131 | |
6 | 32 | 5 | 0.445 | 0.156 | 0.658* | Yes | 0.168 | ||
28 | 33 | 6 | 0.786 | 0.394 | 0.51* | Yes | 0.155 | ||
22 | 33 | 12 | 0.805 | 0.727 | 0.112 | No | 0.112 | ||
23 | 33 | 7 | 0.597 | 0.515 | 0.152 | No | 0.152 | ||
7 | 32 | 7 | 0.754 | 0.219 | 0.718* | Yes | –0.084 | ||
10 | 33 | 13 | 0.865 | 0.576 | 0.348* | Yes | 0.102 | ||
12 | 33 | 7 | 0.648 | 0.333 | 0.498* | Yes | 0.022 | ||
A. pichoni | Kimbe Bay | 2 | 6 | 6 | 0.694 | 0.5 | 0.362 | NED | 0.362 |
6 | 6 | 3 | 0.611 | 0 | 1* | NED | 1 | ||
28 | 6 | 8 | 0.847 | 0.833 | 0.107 | NED | 0.107 | ||
22 | NA | NA | NA | NA | NA | NED | NA | ||
23 | 6 | 4 | 0.514 | 0.5 | 0.118 | NED | 0.118 | ||
7 | 2 | 2 | 0.375 | 0.5 | 0 | NED | 0 | ||
10 | 6 | 3 | 0.542 | 0.167 | 0.737 | NED | 0.737 | ||
12 | 6 | 6 | 0.806 | 0.667 | 0.259 | NED | 0.259 | ||
Truk | 2 | 6 | 6 | 0.806 | 1 | –0.154 | NED | –0.154 | |
6 | 6 | 5 | 0.667 | 0.5 | 0.333 | NED | 0.333 | ||
28 | 6 | 7 | 0.806 | 0.833 | 0.057 | NED | 0.057 | ||
22 | NA | NA | NA | NA | NA | NED | NA | ||
23 | 6 | 2 | 0.375 | 0.5 | –0.25 | NED | –0.25 | ||
7 | 2 | 3 | 0.625 | 0.5 | 0.5 | NED | 0.5 | ||
10 | 5 | 5 | 0.78 | 0.6 | 0.333 | NED | 0.333 | ||
12 | 6 | 6 | 0.806 | 0.333 | 0.643* | NED | 0.643 | ||
A. horrida | Orpheus Island | 2 | 26 | 3 | 0.144 | 0.038 | 0.742* | Yes | 0.059 |
6 | 26 | 8 | 0.803 | 0.346 | 0.582* | Yes | 0.019 | ||
28 | 26 | 10 | 0.686 | 0.346 | 0.51* | Yes | 0.067 | ||
22 | 22 | 8 | 0.834 | 0.364 | 0.579* | Yes | 0.012 | ||
23 | 23 | 4 | 0.427 | 0.565 | –0.303 | No | –0.303 | ||
7 | 17 | 2 | 0.457 | 0 | 1* | Yes | –0.333 | ||
10 | 18 | 4 | 0.335 | 0.389 | –0.133 | No | –0.133 | ||
12 | 18 | 5 | 0.466 | 0.611 | –0.285 | No | –0.285 | ||
A. jacquelineae | Orpheus Island | 2 | 17 | 3 | 0.631 | 0.529 | 0.191 | No | 0.191 |
6 | 17 | 3 | 0.258 | 0.176 | 0.342 | No | 0.342 | ||
28 | 17 | 4 | 0.306 | 0.176 | 0.448 | No | 0.448 | ||
22 | NA | NA | NA | NA | NA | No | NA | ||
23 | 19 | 7 | 0.578 | 0.789 | –0.343 | No | –0.343 | ||
7 | 11 | 3 | 0.657 | 0.545 | 0.216* | No | 0.216 | ||
10 | 11 | 4 | 0.442 | 0.455 | 0.02 | No | 0.02 | ||
12 | 11 | 2 | 0.236 | 0.091 | 0.643 | No | 0.643 | ||
A. kimbeensis | Kimbe Bay | 2 | 13 | 6 | 0.719 | 0.385 | 0.496* | Yes | 0.163 |
6 | 13 | 4 | 0.666 | 0.538 | 0.229 | No | 0.229 | ||
28 | 13 | 10 | 0.858 | 0.615 | 0.319 | Yes | 0.319 | ||
22 | NA | NA | NA | NA | NA | No | NA | ||
23 | 14 | 4 | 0.459 | 0.143 | 0.708* | Yes | 0.071 | ||
7 | 13 | 2 | 0.497 | 0.462 | 0.111 | No | 0.111 | ||
10 | 14 | 7 | 0.694 | 0.857 | –0.2 | No | –0.2 | ||
12 | 14 | 4 | 0.656 | 0.429 | 0.378 | No | 0.378 | ||
A. tortuosa | Rongelap Atoll | 2 | 12 | 4 | 0.462 | 0.25 | 0.492* | Yes | 0.135 |
6 | 12 | 3 | 0.559 | 0.25 | 0.582* | Yes | –0.021 | ||
28 | 12 | 4 | 0.608 | 0.25 | 0.616* | LAD | 0.616 | ||
22 | 12 | 4 | 0.563 | 0.917 | –0.603 | No | –0.603 | ||
23 | 12 | 2 | 0.153 | 0.167 | –0.048 | No | –0.048 | ||
7 | NA | NA | NA | NA | NA | NA | NA | ||
10 | 12 | 2 | 0.278 | 0.333 | –0.158 | No | –0.158 | ||
12 | 12 | 5 | 0.778 | 0.833 | –0.028 | No | –0.028 | ||
A. kirstyae | Orpheus Island | 2 | 24 | 2 | 0.499 | 0.125 | 0.759* | Yes | –0.004 |
6 | 24 | 4 | 0.582 | 0.167 | 0.724* | Yes | –0.129 | ||
28 | 24 | 7 | 0.777 | 0.583 | 0.269 | LAD | 0.269 | ||
22 | 23 | 14 | 0.765 | 0.913 | –0.173 | No | –0.173 | ||
23 | 23 | 7 | 0.433 | 0.348 | 0.218 | No | 0.218 | ||
7 | 22 | 5 | 0.249 | 0.136 | 0.471* | Yes | –0.122 | ||
10 | 23 | 5 | 0.631 | 0.826 | –0.288 | No | –0.288 | ||
12 | 23 | 5 | 0.632 | 0.609 | 0.06* | No | 0.06 | ||
A. spathulata | Orpheus Island | 2 | 26 | 2 | 0.038 | 0.038 | 0* | No | 0 |
6 | 26 | 3 | 0.443 | 0.077 | 0.832* | Yes | –0.098 | ||
28 | 24 | 4 | 0.666 | 0.5 | 0.269* | No | 0.269 | ||
22 | 13 | 4 | 0.388 | 0.154 | 0.628* | Yes | 0.13 | ||
23 | 12 | 2 | 0.153 | 0.167 | –0.048 | No | –0.048 | ||
7 | NA | NA | NA | NA | NA | No | NA | ||
10 | 20 | 8 | 0.8 | 0.9 | –0.1 | No | –0.1 | ||
12 | 22 | 5 | 0.546 | 0.636 | –0.142 | No | –0.142 | ||
A. walindii | Kimbe Bay | 2 | 14 | 2 | 0.408 | 0.571 | –0.368 | NED | –0.368 |
6 | 13 | 2 | 0.426 | 0.154 | 0.662 | NED | 0.662 | ||
28 | 12 | 5 | 0.681 | 0.5 | 0.305 | NED | 0.305 | ||
22 | 14 | 3 | 0.518 | 0.5 | 0.071 | NED | 0.071 | ||
23 | 14 | 1 | 0 | 0 | 0 | NED | 0 | ||
7 | 2 | 1 | 0 | 0 | 0 | NED | 0 | ||
10 | 14 | 3 | 0.135 | 0.143 | –0.02 | NED | –0.02 | ||
12 | 14 | 1 | 0 | 0 | 0 | NED | 0 | ||
A. rongelapensis | Rongelap Atoll | 2 | 12 | 6 | 0.722 | 1 | –0.347* | No | –0.347 |
6 | 12 | 5 | 0.726 | 0.75 | 0.01 | No | 0.01 | ||
28 | 12 | 8 | 0.83 | 0.833 | 0.039* | No | 0.039 | ||
22 | NA | NA | NA | NA | NA | No | NA | ||
23 | 12 | 3 | 0.538 | 1 | –0.846* | No | –0.846 | ||
7 | 12 | 4 | 0.726 | 0.917 | –0.222* | No | –0.222 | ||
10 | 12 | 5 | 0.694 | 0.667 | 0.083 | No | 0.083 | ||
12 | 12 | 5 | 0.674 | 1 | –0.451 | No | –0.451 |