Figure 1.

Dis3 is essential for fly development. (a) Scheme for generating Dis3 RNAi flies with Gal4/UAS-driven transgenic RNAi system. (b) Western blots on nuclear extracts show decrease of Dis3 protein level in Dis3 knock down flies at different larval stages; SNF is used as a loading control. (c) Larval growth of da-Gal4 (left), w1118 (middle) and da-Gal4 x 35090 (right) strain; white bar = 1 mm. (d) Growth curve of da-Gal4, w1118, and da-Gal4 × 35090 fly larvae measured by surface area (mm²). Error bar was standard deviation of measurement of ≥ 30 larva samples. (e) Melanotic masses in da-Gal4 × 35090 fly larvae; black bar = 1 mm.