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Dis3 depletion affects brain growth but does not impact neuronal marker protein distribution or levels. Wild type w1118 and Dis3 RNAi fly larvae were collected at day 5, brains were dissected and stained with (a) anti-Dis3 (green), anti-fasciclin (FAS1; red) or with (b) anti-Dis3 (green) and anti-ELAV (red). Nuclei were stained with DAPI (blue).
