Figure 5.

Dis3 knock down has a pronounced effect on the accumulation of a large number of early developmentally-expressed RNAs. (a) Heatmap of all early expressed RNAs throughout the development in wild type and Dis3KD flies. Phylogenetic analysis presented above the columns reveals how the affected RNAs in the Dis3KD sample are clearly grouped. RNA-seq derived relative expression level of (b) hunchback, (c) Krüppel, and (d) CG12011 during development of WT and Dis3KD flies. Real-time quantitative PCR analysis of (e) hunchback, (f) Krüppel, and (g) CG12011 at the same developmental time points.