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. 2012 May 20;11:63. doi: 10.1186/1475-2859-11-63

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Copyright ©2012 Dashtban and Qin; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Identification of β-glucosidase in BGLI-overexpressing transformants (T1-T4) by MUG-zymogram assay. Proteins (culture supernatant) from BGLI-overexpressing transformants (T1-T4) and the parental strain (T. reesei QM9414, lane QM) were separated in 8% native PAGE. Culture supernatant of Periconia sp. (bgl1 donor strain) (lane P) grown on MA-medium containing 1% microcrystalline was used as the positive control. β-glucosidase activity was detected by MUG-zymogram assay. Lower arrow indicates the native extracellular β-glucosidase found in T. reesei strain (lanes T1-T4 and QM) whereas the upper arrow represents active β-glucosidase correlated to Periconia sp. BGLI only found in the BGLI- overexpressing transformants as well as Periconia sp. (lanes T1-T4 and P). Lane M, molecular weight marker.