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Immunohistochemical staining of a needle biopsy containing an area of prostatic intra-epithelial neoplasia (PIN) with the three-marker cocktail. Prostate glands show staining in both the cytoplasm of luminal epithelial cells (brown granular AMACR-positive) and in the basal epithelial cells (brown nuclear p63-positive as well as red cytoplasm CK5-positive), characteristic for PIN (x 400).
