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. 2012 Sep;140(3):307–324. doi: 10.1085/jgp.201210826

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© 2012 Garg et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 8. — Intragenic rescue of Y464A-enhanced hEAG1 inactivation by a second site mutation in either Phe359 (in S5) or Leu434 (in pore helix). (A–E) Currents for indicated mutant hEAG1 channels recorded during two-pulse inactivation protocol: V_h = −100 mV, and V_pre (10 s) ranged from −130 to +20 mV, applied in 15-mV increments. A test pulse to +30 mV was applied after each V_pre. The top set of traces in each panel is for channels with indicated single mutation; the bottom set of traces shows currents for same mutation introduced into the Y464A channel. All mutations except the conserved F359Y (A, bottom) and L434M (C, bottom) rescued inactivation of Y464A. Inward currents in some traces represent unsubtracted leak currents. Based on reversal potential of time-dependent currents, only F359L/Y464A channels had reduced K⁺ selectivity. Currents shown are representative of four to six oocytes for each mutant channel.