Figure 6.

TPeA block of EMD 41000–activated and ryanodine-modified WT and substituted channels. The top panel shows representative traces of RyR2 in the absence of TPeA after addition of 20 µM EMD 41000 (left) and 1 µM ryanodine (right). The bottom left panel shows block of the WT and alanine-substituted EMD 41000–responsive channels by 100 µM TPeA at 60 mV. Closed levels are indicated by arrows. The bottom right panel shows representative traces of block of WT and substituted channels by 100 µM TPeA at 60 mV after modification by 1 µM ryanodine.