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. 2012 Sep 5;7(9):e44312. doi: 10.1371/journal.pone.0044312

Figure 4. Evaluation of P-glycoprotein activity.

Figure 4

To measure P-gp activity the substrate Rhodamine 123 was used as substrate. LMW-PTP was silenced and after 24 h cells were further incubated for 1 h in the presence of 200 ng/mL Rhodamine 123 with or without a P-gp inhibitor (5 µM verapamil). Afterwards, cells were washed with PBS and Rhodamine 123 mean fluorescence intensity was assessed by flow cytometry analysis within the live-gate, using a FACScalibur (Becton and Dickinson, USA). Decreasing of mean fluorescence intensity indicates higher P-gp activity.