Figure 1. ppkl expression and activity.

A. Schematic representation of PPKL structure showing N-terminal Kelch repeats and C-terminal catalytic domain. Residues of the first motif of the catalytic domain are indicated B. Phosphatase assay demonstrates PPKL is an active phosphatase whereas the catalytic site mutant (-GAXNE-) shows no activity. C. RNA-Seq data represented as both tags/kb of gene and fragments per kilobase of exon model per million mapped reads (FPKM) indicates ppkl mRNA levels are highest in schizonts and gametocytes D. Immunoblots of parasite extracts from rings, trophozoite, schizont stages from a gametocyte non-producer and, gametocyte and ookinete stages using a α-PPKL polyclonal antibody. Protein expression is detected only in schizonts and sexual stages.