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. 2012 Sep 5;7(9):e44096. doi: 10.1371/journal.pone.0044096

Figure 3. Overexpression of Axin1Q162A blocks axis formation and Wnt signaling in Xenopus embryos.

Figure 3

(A–C) Representative phenotypes of stage 40 control uninjected embryos (A), axin1-injected embryos (500 pg), (B) and axin1(Q162A)-injected embryos (500 pg) (C). (D) Representative realtime RT-PCR of sia1, nr3.1 and chd expression in control whole embryos (un WE), control animal caps (un cap) and caps injected with wnt8 (1 pg) and Axin constructs (500 pg). (E) Realtime RT-PCR of nr3.1 expression in animal caps injected with wnt8 and different doses of Axin constructs (100 pg, 300 pg, 500 pg; indicated below sets of bars).