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. Author manuscript; available in PMC: 2013 Sep 5.
Published in final edited form as: J Am Chem Soc. 2012 Aug 21;134(35):14580–14594. doi: 10.1021/ja3058474

Figure 2.

Figure 2

Dependence of the observed second-order rate constant kobsd/[E] (M−1 s−1) for exchange for deuterium of the C-6 proton of F-UMP catalyzed by ScOMPDC (0.22 – 14 μM) on the total concentration of F-UMP in D2O at pD 8.15, 25 °C and I = 0.10 (NaCl). The solid line shows the fit of the data to eq 4, which gives values of kex = 0.048 s−1 and Kd = 0.14 mM.