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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: J Neurochem. 2012 Jun 6;122(2):295–307. doi: 10.1111/j.1471-4159.2012.07787.x

Fig. 4.

Fig. 4

Overexpression of NR2A and NR2B enhances NMDA-induced phosphorylation at Thr286. Cortical neurons were transfected with plasmid expressing GFP or GFP-NR2A or GFP-NR2B at DIV 12. At DIV 16, non-stimulated neurons (A) or NMDA (20 μM for 10 min)-stimulated neurons (B) were fixed and co-stained for phosphorylated CaMKIIα (at Thr286) and GFP. The level of Thr286 phosphorylation in representative neurons transfected with GFP, or GFP-NR2A, or GFP-NR2B (as indicated by the arrows) is shown in A1 and B1. For quantifications shown in A2 and B2, the level of Thr286 phosphorylation in neurons transfected with GFP was defined as 1, and the immuno-intensity in neurons transfected with GFP-NR2A or GFP-NR2B was normalized to the GFP controls. The quantification is presented as average +/− SEM. *: p < 0.05, by ANOVA.