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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: J Neurochem. 2012 Jun 6;122(2):295–307. doi: 10.1111/j.1471-4159.2012.07787.x

Fig. 6.

Fig. 6

Overexpression of NR2A or NR2B exaggerates CaMKIIα dephosphorylation after stimulation with high concentration of NMDA. Cortical neurons were transfected with plasmid expressing GFP or GFP-NR2A or GFP-NR2B at DIV 12. At DIV 16, neurons were first pre-treated with TTX (1 μM), CNQX (20 μM) and nifedipine (5 μM), and stimulated with 80 μM NMDA for 10 min. Neurons were then fixed and co-stained for phosphorylated CaMKIIα and GFP. The level of Thr286 phosphorylation in representative neurons transfected with GFP, or GFP-NR2A, or GFP-NR2B (as indicated by the arrows) is shown in A. The quantification is presented as average +/− SEM in B. *: p < 0.05, by ANOVA.