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. 2012 Apr 10;22(9):1374–1389. doi: 10.1038/cr.2012.57

Figure 6.

Figure 6

WDR83 and DHPS were involved in the regulation of cell proliferation, and the downregulation of WDR83 or DHPS inhibited ERK1/2 activation and E2F1 expression in MGC803 cells. (A) The knockdown of either WDR83 or DHPS decreased PMA-stimulated ERK1/2 phosphorylation compared with negative controls in MGC803 cells. (B) Overexpression of WDR83 or DHPS in combination with PMA enhanced ERK1/2 phosphorylation. (C) The knockdown of WDR83, DHPS, or ERK1/2 resulted in a significant decrease in E2F1 protein levels compared with the control cells. (D) Overexpression of WDR83 or DHPS induced an increase in E2F1 protein levels. (E) Downregulation of WDR83 or DHPS significantly inhibited cell proliferation in MGC803 cells (P ≤ 0.0001 and P = 0.0006, respectively). (F, G) In contrast, the overexpression of WDR83 or DHPS enhanced cell proliferation (P = 0.0148 and P = 0.0003, respectively). (H) Interestingly, the joint overexpression of WDR83 and DHPS enhanced cell proliferation even further (P = 0.0037). Cell proliferation was determined by the CCK-8 assay (n ≥ 3, ANOVA; *P< 0.05, **P< 0.01, ***P< 0.001).