Figure 2. Differential profiles of SPM and their biosynthetic pathway markers in inflammatory exudates.

Lavage exudates (3 mL) collected in 5 mL lavages from the peritoneum at 4 hrs were subjected to LC-MS-MS lipidomics. Bioactive lipid mediators and precursor/pathway marker were identified using previously established criteria²⁶. (a,b) Representative tandem mass spectra of LTB₄ and PD1 employed for identification. (c–l) Quantification was achieved by multiple reaction monitoring of Q1: M–H (parent ion), Q3: diagnostic ion in the MS-MS (daughter ion). (d–f) 4 hr, (g-i) 12 hr , (j–l) 24 hr exudates. (c) Ratios of pro-resolving versus pro-inflammatory mediators. Results are mean ± SEM, n = 4 separate murine exudates. *P<0.05, **P<0.01, ***P<0.001, self-limited versus delayed resolution challenge. Black bars, self-limited; Gray bars, delayed resolution.