Figure 5. Apoptotic PMN stimulate resolution programs during acute inflammation.

At 12 hrs, apoptotic human PMN (5×10⁶ PMN/mouse) were injected into mouse peritonitis exudates initiated by zymosan (1 mg or 10 mg/mouse; see Fig. 1) injection. Exudates were harvested at 24 hours. (a) miR-219-2-3p and (b) 5-LOX expression were quantified via qPCR. (A, inset) Representative macrophage and PMN from exudate (I) bright field, (II) DAPI, (III) Merged image. Magnification 40X. Representative of n = 4 separate murine exudates. (c–f) Bioactive lipid mediators and precursor/pathway markers were identified and quantified (see Materials and Methods). Results are mean ± SEM of n = 4 separate murine exudates. *P<0.05, **P<0.01. Self-limited zymosan vs. zymosan plus apoptotic PMN. #p<0.05 delayed resolution zymosan vs. zymosan plus apoptotic PMN. §P<0.05 self-limited zymosan versus delayed resolution zymosan challenge. Black bars, self-limited; Gray bars, delayed resolution. Z: zymosan, AP: apoptotic PMN.