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. 2012 Sep 6;2:637. doi: 10.1038/srep00637

Figure 6. ISGF3-ISRE DNA binding activity is increased in miR122-silenced cells.

Figure 6

(a) DNA-binding ability of ISGF3 was determined by gel shift assay using ISRE consensus oligonucleotides. Nuclear extracts from control and miR122-silenced Huh7 cells with and without IFN-α stimulation for 1 h were used. Arrow indicates ISGF3-DNA complexes. The specificity of the DNA–protein complex was tested by adding unlabeled ISRE probe (cold probe) to IFN-α-stimulated miR122-silenced Huh7 nuclear extracts. A representative of three independent determinations is shown. (b) DNA-binding ability of Oct-1 was determined as a control for equal loading of nuclear extract. (c) TFIID amounts were examined by Western blotting to confirm equal nuclear extract loading. A representative of two independent determinations is shown.