Figure 3.

Characterization of fluorescent coagulation factor probe binding to MDA-MB-231 and SW620 cells in human plasma. One-hundred thousand MDA-MB-231 (A) or SW620 cells (B) were incubated with vehicle or fluorescently modified, active site-inhibited coagulation factors FVIIa (10–500 nM), FXa (100–5000 nM), or FIIa (100–10,000 nM) in 50 μL of PPP for 30 min at room temperature. Cells were then diluted 10-fold in sterile-filtered phosphate-buffered saline (PBS, pH = 7.40) and analyzed by flow cytometry. Shaded histograms represent background fluorescence while white histograms represent labeled cells at the fluorescent probe concentrations shown.