Figure 6.

Characterization of fluorescent coagulation factor probe binding to immobilized MDA-MB-231, SW480, and SW620 cells in the presence of coagulation. MDA-MB-231 (A), SW480 (B), and SW620 cells (C) were immobilized on silanized glass slides and incubated with recalcified human plasma containing the anti-FXIa antibody, 1A6, and the fibrin polymerization inhibitor, GPRP, for 30 min at room temperature. Cells were then washed and treated with PBS (vehicle) or PBS containing fluorescently labeled coagulation factors FVIIa (500 nM), FXa (5 μM), or FIIa (10 μM) for 30 min at room temperature, washed in PBS, and fixed in 3.7% paraformaldehyde. Immobilized cells were imaged using differential interference contrast (DIC) and fluorescence microscopy.