Figure 8.

Characterization of fluorescent coagulation factor probe binding to immobilized human neutrophils and platelets. Human platelets (A) and neutrophils (B) were immobilized on silanized glass slides and incubated with PBS (vehicle) containing fluorescently labeled coagulation factors FVIIa (500 nM), FXa (5 μM), or FIIa (10 μM) for 30 min at room temperature, washed in PBS and fixed in 3.7% paraformaldehyde. Immobilized cells were imaged using differential interference contrast (DIC) and fluorescence microscopy.