Fig 3.

Validation of the Btk-Liar interaction. (A) Coimmunoprecipitation validation of ectopic expression of Liar (DsRed-Liar-Myc) with pSGT-Btk in Cos-7 cells; (B) endogenous interaction of Btk and Liar in Namalwa B cells; (C) influence of Btk phosphorylation status on Liar interaction using the Btk inhibitor PCI-32765. Cells were activated by pervanadate; (D) influence of phosphomimetic mutations Y223D and Y551D on Liar interaction. Lane 1, untransfected control (other controls were mock transfected); lane 2, cells transfected with pEGFP plasmid only; lane 3, cells transfected with pSGT empty vector only.