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Liar is a nucleocytoplasmic protein colocalizing with Btk in the cytoplasm, inner membrane, and perinuclear regions. (A) Liar localization with anti-Myc staining enhanced with Cy3. GFP was expressed together with Liar using the pMSCV-Liar-IRES-GFP plasmid. (B) DsRed-Liar expressed alone. (C) Btk-GFP expressed alone. (D) Btk-GFP coexpressed with DsRed-Liar. Equal amounts of plasmids were transiently transfected in Cos7, and colocalization was monitored 48 h posttransfection.
